Recombinant Mouse Pofut2 Protein, Myc/DDK-tagged

• Cat.No.: Pofut2-4979M
• Product Overview: Purified recombinant protein of mouse full-length protein O-fucosyltransferase 2 (Pofut2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X2-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13. O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 49.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pofut2 protein O-fucosyltransferase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Pofut2
• Synonyms: POFUT2; protein O-fucosyltransferase 2; GDP-fucose protein O-fucosyltransferase 2; O-FucT-2; peptide-O-fucosyltransferase 2; FUT13; AI256847; BC003494; C21orf80; MGC6943; 2310011G23Rik; MGC25565
• Gene ID: 80294
• mRNA Refseq: NM_030262
• Protein Refseq: NP_084538
• UniProt ID: Q8VHI3