Recombinant Mouse Polr1e Protein, Myc/DDK-tagged

• Cat.No.: Polr1e-4995M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 54.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Polr1e polymerase (RNA) I polypeptide E [ Mus musculus (house mouse) ]
• Official Symbol: Polr1e
• Synonyms: POLR1E; polymerase (RNA) I polypeptide E; DNA-directed RNA polymerase I subunit RPA49; RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53; polymerase (RNA) I associated factor 1; DNA-directed RNA polymerase I subunit E; RNA polymerase I associated factor (Paf53); 53kDa; Paf53; Praf1; AU042259; D030019D19Rik
• Gene ID: 64424
• mRNA Refseq: NM_022811
• Protein Refseq: NP_073722
• UniProt ID: Q8K202