Recombinant Mouse Pou5f1 Protein, His-tagged

• Cat.No.: Pou5f1-640M
• Product Overview: Recombinant Mouse Pou5f1(Ser129~Asn352) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein plays a key role in embryonic development and stem cell pluripotency. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 29.2kDa
• Identity: Reconstitute in PBS or others
• Protein length: Ser129~Asn352
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: Pou5f1 POU domain, class 5, transcription factor 1 [ Mus musculus ]
• Official Symbol: Pou5f1
• Synonyms: POU5F1; POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7
• Gene ID: 18999
• mRNA Refseq: NM_001252452
• Protein Refseq: NP_001239381
• UniProt ID: P20263