Recombinant Mouse Ppid Protein, His-tagged

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Recombinant Mouse Ppid Protein, His-tagged

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Cat.No. : Ppid-32M
Product Overview : Recombinant Mouse Ppid Protein (1-370 aa) is produced by E. coli expression system. This protein is fused with a His-tag.
Description : Ppid, also known as Peptidyl-prolyl cis-trans isomerase D, is a member of peptidyl-propyl cis-trans isomerase (Pplase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression the apoptosis in caner cells. Recombinant mouse Ppid protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Source : E. coli
Species : Mouse
Tag : His
Form : Liquid. In 20mM Tris-Hcl buffer (pH8.0) containing 10% glycerol, 1mM DTT
Bio-activity : Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Molecular Mass : 43.4 kDa (395aa) confirmed by MALDI-TOF
Protein length : 1-370 aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSEFMSHAS PAAKPSNSKN PRVFFDVDIG GERVGRIVLE LFADIVPKTA ENFRALCTGE KGTGSTTGKP LHFKGCPFHR IIKKFMIQGG DFSNQNGTGG
ESIYGEKFED ENFHYKHDRE GLLSMANAGP NTNGSQFFIT TVPTPHLDGK HVVFGQVIKG LGVARTLENV EVNGEKPAKL CVIAECGELK EGDDWGIFPK DGSGDSHPDF PEDADIDLKD
VDKILLISED LKNIGNTFFK SQNWEMAIKK YAKVLRYVDS SKAVIEKADR SRLQPIALSC VLNIGACKLK MSNWQGAIDS CLEALEMDPS NTKALYRKAQ GWQGLKEYDQ ALADLKKAQE IAPGDKAIQA ELLKVKQMIK AQKDKEKAVY AKMFA
Purity : > 90% by SDS - PAGE
Applications : Enzyme Activity; SDS-PAGE
Notes : For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage : Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Absorbance at 280nm)
Figure :

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Gene Name : Ppid peptidylprolyl isomerase D (cyclophilin D) [ Mus musculus ]
Official Symbol : Ppid
Synonyms : PPID; PPIase D; rotamase D; cyclophilin 40; Ppif; Ppidl; CYP-40; 4930564J03Rik;
Gene ID : 67738
mRNA Refseq : NM_026352
Protein Refseq : NP_080628
UniProt ID : Q9CR16

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