Recombinant Mouse Prkca protein, His & S-tagged

• Cat.No.: Prkca-1971M
• Product Overview: Recombinant Mouse Prkca aa. (Phe339~Phe597 (Accession # P20444)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His & S
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 35.2 kDa
• Protein length: Phe339~Phe597 (Accession # P20444)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 5.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Prkca protein kinase C, alpha [ Mus musculus (house mouse) ]
• Official Symbol: Prkca
• Synonyms: Cyp40; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; P450C1 alpha; P450VD1-alpha; VD3 1A hydroxylase
  calcidiol 1-monooxygenase; cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1; cytochrome P450 subfamily XXVIIB polypeptide 1; cytochrome P450, 40 (25-hydroxyvitamin D3 1 alpha-hydroxylase); cytochrome P450, subfamily 27b, polypeptide 1; cytochrome P450C1 alpha; cytochrome P450VD1-alpha; cytochrome p450 27B1; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
• Gene ID: 18750
• mRNA Refseq: NM_011101.3
• Protein Refseq: NP_035231.2
• UniProt ID: P20444