Recombinant Mouse Psmd10 Protein, Myc/DDK-tagged

• Cat.No.: Psmd10-5189M
• Product Overview: Purified recombinant protein of mouse full-length proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.
• Molecular Mass: 25.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Psmd10 proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 [ Mus musculus (house mouse) ]
• Official Symbol: Psmd10
• Synonyms: PSMD10; proteasome (prosome, macropain) 26S subunit, non-ATPase, 10; 26S proteasome non-ATPase regulatory subunit 10; gankyrin; proteasome subunit p28; proteosome subunit p28; 26S proteasome regulatory subunit p28; proteosome (prosome, macropain) 26S subunit, non-ATPase, 10; AW554874
• Gene ID: 53380
• mRNA Refseq: NM_016883
• Protein Refseq: NP_058579
• UniProt ID: Q9Z2X2