Recombinant Mouse Psme3 Protein, Myc/DDK-tagged

• Cat.No.: Psme3-5203M
• Product Overview: Purified recombinant protein of mouse full-length proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) (Psme3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 29.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Psme3 proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) [ Mus musculus (house mouse) ]
• Official Symbol: Psme3
• Synonyms: PSME3; proteaseome (prosome, macropain) 28 subunit, 3; proteasome activator complex subunit 3; REG-gamma; ki nuclear autoantigen; 11S regulator complex subunit gamma; proteasome activator 28 subunit gamma; activator of multicatalytic protease subunit 3; Ki; pa28g; AA410043; AU020960; REGgamma; PA28gamma
• Gene ID: 19192
• mRNA Refseq: NM_011192
• Protein Refseq: NP_035322
• UniProt ID: P61290