Recombinant Mouse Ptn protein(Gly33-Asp168), hFc-tagged
Cat.No. : | Ptn-7414M |
Product Overview : | Recombinant Mouse PTN (P63089) (Gly 33-Asp 168) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | N-hFc |
Protein length : | Gly33-Asp168 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant mouse PTN/Fc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a calculated molecular mass of 43.7 kDa. The apparent molecular mass of the monomer is approximately 47 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 85 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Ptn pleiotrophin [ Mus musculus ] |
Official Symbol : | Ptn |
Synonyms : | PTN; pleiotrophin; HBBM; osteoblastic cell factor; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin-binding growth factor 8; heparin affin regulatory peptide; heparin-binding neutrophic factor; heparin-binding neurotorphic factor; heparin-binding neurite promoting factor; heparin-binding growth-associated molecule; OSF; HARP; HBBN; HBNF; Osf1; Osf-1; HB-GAM; HBGF-8; |
Gene ID : | 19242 |
mRNA Refseq : | NM_008973 |
Protein Refseq : | NP_032999 |
Products Types
◆ Recombinant Protein | ||
PTN-261H | Recombinant Human PTN Protein (Gly33-Asp168), C-His tagged, Animal-free, Carrier-free | +Inquiry |
PTN-3514R | Recombinant Rhesus Macaque PTN Protein, His (Fc)-Avi-tagged | +Inquiry |
PTN-6088H | Active Recombinant Human PTN Protein(Gly33~Asp168), His-tagged | +Inquiry |
Ptn-2001R | Recombinant Rat Ptn Protein, His-tagged | +Inquiry |
PTN-232H | Recombinant Human PTN Protein | +Inquiry |
◆ Lysates | ||
PTN-001HCL | Recombinant Human PTN cell lysate | +Inquiry |
PTN-001MCL | Recombinant Mouse PTN cell lysate | +Inquiry |
PTN-1524MCL | Recombinant Mouse PTN cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionPTN plays a critical role in neurodevelopment and neuronal plasticity. During embryonic development, PTN promotes axonal outgrowth, neuronal migration, and synaptogenesis. It interacts with cell surface receptors on neurons, such as ALK, to activate downstream signaling pathways that modulate cytoskeletal dynamics and promote neurite growth. In the adult brain, PTN is involved in synaptic plasticity, memory formation, and neuronal survival. Dysregulation of PTN expression or signaling can disrupt normal neurodevelopmental processes and contribute to neurological disorders.
PTN has shown promise as a diagnostic biomarker for certain diseases. Increased PTN expression has been observed in the serum or tissue samples of patients with various cancers, suggesting its potential as a cancer biomarker. Additionally, PTN levels in cerebrospinal fluid have been associated with neurodegenerative disorders, including Alzheimer's disease. Ongoing research aims to develop sensitive and specific assays for PTN detection, which could aid in early disease diagnosis, prognosis, and monitoring of treatment response in these conditions.
PTN binding to its cell surface receptors initiates downstream signaling pathways that regulate cellular functions. One of the major pathways activated by PTN is the phosphoinositide 3-kinase (PI3K)/Akt pathway, which promotes cell survival and growth. PTN can also activate the mitogen-activated protein kinase (MAPK) pathway, including extracellular signal-regulated kinase (ERK), leading to cellular proliferation and differentiation. Additionally, PTN can influence intracellular calcium signaling and modulate the activity of various transcription factors, ultimately impacting gene expression and cellular behavior.
The protein PTN, also known as pleiotrophin, plays a crucial role in various biological processes. It functions as a cytokine and growth factor that regulates cellular proliferation, differentiation, and migration. PTN is involved in embryonic development, tissue repair, and the maintenance of neuronal plasticity. Its interactions with cell surface receptors, such as receptor protein tyrosine phosphatase beta/zeta (RPTPβ/ζ) and anaplastic lymphoma kinase (ALK), mediate downstream signaling cascades that influence cellular behavior and tissue homeostasis.
Dysregulation of PTN expression has been implicated in several diseases. Increased PTN levels have been observed in various types of cancer, including breast, lung, and glioblastoma, where it promotes tumor growth, angiogenesis, and metastasis. Furthermore, aberrant PTN expression has been linked to neurodegenerative disorders, such as Alzheimer's disease, where it contributes to neuronal dysfunction and neuroinflammation. In cardiovascular diseases, PTN can promote vascular smooth muscle cell proliferation and contribute to the development of atherosclerosis.
The transcriptional regulation of the PTN gene involves complex mechanisms. Several transcription factors, including AP-1, NF-κB, and SP1, have been identified as regulators of PTN expression. Additionally, epigenetic modifications, such as DNA methylation and histone acetylation, can modulate PTN gene activity. Furthermore, various extracellular stimuli, including growth factors and cytokines, can induce PTN expression through the activation of specific signaling pathways, such as the MAPK/ERK and PI3K/Akt pathways.
Inhibition of PTN activity or downstream effectors is being investigated as a potential therapeutic approach. Small molecule inhibitors and monoclonal antibodies targeting PTN or its receptors are being developed to block PTN signaling and mitigate its pathological effects. Additionally, strategies to modulate PTN expression through gene therapy or targeted delivery of RNA-based therapeutics are being explored. The efficacy of these approaches is being evaluated in preclinical and clinical studies to determine their potential for treating PTN-related diseases.
Customer Reviews (3)
Write a reviewBy utilizing this experimental reagent, I save a significant amount of time and effort, enabling a seamless workflow.
Using this reagent, I can rapidly obtain high-quality experimental data.
Following the implementation of this protein reagent, the reproducibility of my experiments has significantly improved, leaving me highly satisfied.
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