Recombinant Mouse Pus10 Protein, Myc/DDK-tagged

• Cat.No.: Pus10-5259M
• Product Overview: Purified recombinant protein of mouse full-length pseudouridylate synthase 10 (Pus10), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 59.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pus10 pseudouridylate synthase 10 [ Mus musculus (house mouse) ]
• Official Symbol: Pus10
• Synonyms: PUS10; pseudouridylate synthase 10; putative tRNA pseudouridine synthase Pus10; psi55 synthase; tRNA-uridine isomerase; tRNA pseudouridylate synthase; tRNA pseudouridine 55 synthase; coiled-coil domain containing 139; coiled-coil domain-containing protein 139; C77560; Ccdc139; AU014648; 2810013G11Rik; 4933435A13Rik; RP23-164N15.2
• Gene ID: 74467
• mRNA Refseq: NM_028304
• Protein Refseq: NP_082580
• UniProt ID: Q9D3U0