Recombinant Mouse Pym1 Protein, Myc/DDK-tagged

• Cat.No.: Pym1-5274M
• Product Overview: Purified recombinant protein of mouse full-length PYM homolog 1, exon junction complex associated factor (Pym1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions.
• Molecular Mass: 22.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pym1 PYM homolog 1, exon junction complex associated factor [ Mus musculus (house mouse) ]
• Official Symbol: Pym1
• Synonyms: Pym1; PYM homolog 1, exon junction complex associated factor; Py; Pym; Wib; Wibg; A030010B05Rik; partner of Y14 and mago; protein wibg homolog; within bgcn homolog
• Gene ID: 78428
• mRNA Refseq: NM_001170869
• Protein Refseq: NP_001164340
• UniProt ID: Q8CHP5