Recombinant Mouse Retsat Protein, Myc/DDK-tagged

• Cat.No.: Retsat-5473M
• Product Overview: Purified recombinant protein of mouse full-length retinol saturase (all trans retinol 13,14 reductase) (Retsat), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol. Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers. May play a role in the metabolism of vitamin A. Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP. Required for adipocyte differentiation in a 3T3-L1 cell culture model. This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 67.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Retsat retinol saturase (all trans retinol 13,14 reductase) [ Mus musculus (house mouse) ]
• Official Symbol: Retsat
• Synonyms: RETSAT; retinol saturase (all trans retinol 13,14 reductase); all-trans-retinol 13,14-reductase; all-trans-13,14-dihydroretinol saturase; PPAR-alpha-regulated and starvation-induced gene protein; MMT-7; Ppsig; C80029; 0610039N19Rik; MGC143538
• Gene ID: 67442
• mRNA Refseq: NM_026159
• Protein Refseq: NP_080435
• UniProt ID: Q64FW2