Recombinant Mouse Rnf114 Protein, Myc/DDK-tagged

• Cat.No.: Rnf114-5544M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 114 (Rnf114), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Acts also as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 26.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnf114 ring finger protein 114 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf114
• Synonyms: RNF114; ring finger protein 114; RING finger protein 114; zinc finger protein 313; Zfp228; Zfp313; Znf228; AI225886; AW549494; 1110008J21Rik
• Gene ID: 81018
• mRNA Refseq: NM_030743
• Protein Refseq: NP_109668
• UniProt ID: Q9ET26