Recombinant Mouse Rnf4 protein, His & GST-tagged Rnf4-1654M

Recombinant Mouse Rnf4 protein, His & GST-tagged


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Recombinant Mouse Rnf4 protein, His & GST-tagged

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Cat.No. : Rnf4-1654M
Product Overview : Recombinant Mouse Rnf4 aa. (Met1~Ile194 (Accession # Q9QZS2)) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : This gene encodes a small nuclear RING finger protein that mediates ubiquitylation of polysumoylated proteins. Deficiency of the encoded protein in mice leads to embryonic lethality and global DNA hypermethylation. A similar protein in humans is required for arsenic-induced degradation of promyelocytic leukemia protein in acute promyelocytic leukemia. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on chromosome 10.
Source : E. coli
Species : Mouse
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 51.9kDa
Protein length : Met1~Ile194 (Accession # Q9QZS2)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >90%
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in sterile ddH2O.
Isoelectric Point : 7.6

Gene Name : Rnf4 ring finger protein 4 [ Mus musculus (house mouse) ]
Official Symbol : Rnf4
Synonyms : Rnf4; ring finger protein 4; SNURF; Gtrgeo8; AU018689; E3 ubiquitin-protein ligase RNF4; E3 ubiquitin ligase RNF4; RING-type E3 ubiquitin transferase RNF4
Gene ID : 19822
mRNA Refseq : NM_001304269.1
Protein Refseq : NP_001291198.1
UniProt ID : Q9QZS2

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