Recombinant Mouse Rock2 Protein, His-tagged

• Cat.No.: Rock2-2025M
• Product Overview: Recombinant Mouse Rock2 Protein (Leu904-Gly1140) with N-His tag was expressed in E. coli.

Specification

• Description: Enables protein serine/threonine kinase activity. Involved in several processes, including positive regulation of macromolecule metabolic process; protein phosphorylation; and regulation of signal transduction. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway via death domain receptors; nervous system development; and positive regulation of centrosome duplication. Located in Schaffer collateral - CA1 synapse; glutamatergic synapse; and spindle pole centrosome. Is expressed in several structures, including central nervous system; genitourinary system; heart; musculature; and somite. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.0 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Leu904-Gly1140
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Rock2 Rho-associated coiled-coil containing protein kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Rock2
• Synonyms: ROCK2; Rho-associated coiled-coil containing protein kinase 2; rho-associated protein kinase 2; p164 ROCK-2; Rho-associated coiled-coil forming kinage 2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II; Rock2m; Rock-II; ROKalpha; mKIAA0619; Rho-kinase; B230113H15Rik
• Gene ID: 19878
• mRNA Refseq: NM_009072
• Protein Refseq: NP_033098
• UniProt ID: P70336

Reviews

08/19/2021

Swift and hassle-free delivery ensured our experiments stayed on track.

01/13/2020

Efficient customer service provided valuable insights for our decision-making.

07/06/2019

Reliable and precise—this recombined protein never disappoints.

Q&As

Discuss the role of ROCK2 in smooth muscle contraction and vascular tone.

ROCK2 is a key regulator of smooth muscle contraction, influencing vascular tone by phosphorylating myosin light chain and promoting actin-myosin interaction.

Describe the molecular crosstalk between ROCK2 and other signaling pathways.

ROCK2 interacts with various signaling pathways, including Wnt, PI3K/Akt, and MAPK pathways, forming a network that regulates cell survival, proliferation, and differentiation.

How does ROCK2 contribute to the regulation of cell cycle progression?

ROCK2 can influence the cell cycle by regulating cyclin-dependent kinase activity and promoting the phosphorylation of proteins involved in cell cycle checkpoints.

What are the implications of ROCK2 in cell migration and invasion?

ROCK2 is involved in the regulation of cell migration and invasion by controlling actin dynamics and focal adhesion turnover, influencing processes like metastasis in cancer.

Discuss the role of ROCK2 in the regulation of the actin cytoskeleton.

ROCK2 phosphorylates various downstream targets, such as myosin light chain, leading to actin-myosin contraction and the reorganization of the actin cytoskeleton.