Recombinant Mouse Rpe65 Protein, His-tagged

• Cat.No.: Rpe65-2031M
• Product Overview: Recombinant Mouse Rpe65 Protein (Ser2-Ser533) with N-His tag was expressed in E. coli.

Specification

• Description: Enables retinal isomerase activity. Acts upstream of or within several processes, including cellular response to electrical stimulus; insulin receptor signaling pathway; and retinal metabolic process. Located in cytoplasm; nucleus; and plasma membrane. Is expressed in eye; pelvic urothelial lining; pigmented retinal epithelium; and urothelium of ureter. Used to study Leber congenital amaurosis 2 and retinitis pigmentosa 20. Human ortholog(s) of this gene implicated in Leber congenital amaurosis 2; retinitis pigmentosa; retinitis pigmentosa 20; and retinitis pigmentosa 87. Orthologous to human RPE65 (retinoid isomerohydrolase RPE65).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 64.7 kDa
  Accurate Molecular Mass: 65 kDa
• Protein length: Ser2-Ser533
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Rpe65 retinal pigment epithelium 65 [ Mus musculus (house mouse) ]
• Official Symbol: Rpe65
• Synonyms: RPE65; retinal pigment epithelium 65; retinoid isomerohydrolase; retinol isomerase; modifier of retinal degeneration 1; retinal pigment epithelium, 65 kDa; all-trans-retinyl-palmitate hydrolase; retinal pigment epithelium-specific 65 kDa protein; LCA2; RP20; rd12; 65kDa; Mord1; A930029L06Rik
• Gene ID: 19892
• mRNA Refseq: NM_029987
• Protein Refseq: NP_084263
• UniProt ID: Q91ZQ5