Recombinant Mouse Rufy4 Protein, Myc/DDK-tagged

• Cat.No.: Rufy4-5649M
• Product Overview: Purified recombinant protein of mouse full-length RUN and FYVE domain containing 4 (Rufy4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.
• Molecular Mass: 64 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rufy4 RUN and FYVE domain containing 4 [ Mus musculus (house mouse) ]
• Official Symbol: Rufy4
• Synonyms: RUFY4; RUN and FYVE domain containing 4; RUN and FYVE domain-containing protein 4; F930048N03Rik
• Gene ID: 435626
• mRNA Refseq: NM_001170641
• Protein Refseq: NP_001164112
• UniProt ID: Q3TYX8