Recombinant Mouse S100b, His-tagged

• Cat.No.: S100b-2571M
• Product Overview: Recombinant mouse S100B protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 12.8 kDa (112aa).

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDEDGDGECD FQEFMAFVAM VTTACHEFFE HE
• Purity: > 85% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  The receptor for advanced glycation endproducts mediates podocyte heparanase expression through NF-κB signaling pathway (2018)
  Circulating Brain Microvascular Endothelial Cells (cBMECs) as Potential Biomarkers of the Blood–Brain Barrier Disorders Caused by Microbial and Non-Microbial Factors (2013)

Gene Information

• Gene Name: S100b S100 protein, beta polypeptide, neural [ Mus musculus ]
• Synonyms: S100b; S100 protein, beta polypeptide, neural; Bpb; AI850290; MGC74317; protein S100-B; OTTMUSP00000021974; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B
• Gene ID: 20203
• mRNA Refseq: NM_009115
• Protein Refseq: NP_033141
• UniProt ID: P50114
• Chromosome Location: 10 C1; 10 41.0 cM
• Function: calcium ion binding; metal ion binding; receptor binding