Recombinant Mouse Sae1 Protein, Myc/DDK-tagged

• Cat.No.: Sae1-5678M
• Product Overview: Purified recombinant protein of mouse full-length SUMO1 activating enzyme subunit 1 (Sae1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
• Molecular Mass: 38.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sae1 SUMO1 activating enzyme subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: Sae1
• Synonyms: SAE1; SUMO1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; SUMO-1 activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme subunit 1; AOS1; Sua1; Uble1a; HSPC140; AL033372; AW743391; D7Ertd177e; 2400010M20Rik; 2610044L12Rik
• Gene ID: 56459
• mRNA Refseq: NM_019748
• Protein Refseq: NP_062722
• UniProt ID: Q9R1T2