Recombinant Mouse Sema3a protein, His-tagged

• Cat.No.: Sema3a-7972M
• Product Overview: Recombinant Mouse Sema3a protein(O08665)(Tyr21-Val772), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Tyr21-Val772
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 100mM L-Arginine, 100mM glutamic acid (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a predicted MW of 86.6 kDa same as Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Mus musculus ]
• Official Symbol: Sema3a
• Synonyms: SEMA3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III; SemD; SEMA1; Semad; coll-1; Hsema-I
• Gene ID: 20346
• mRNA Refseq: NM_001243072
• Protein Refseq: NP_001230001