Recombinant Mouse Serpinf2 protein(Met1-Lys491), His-tagged

• Cat.No.: Serpinf2-853M
• Product Overview: Recombinant Mouse Serpinf2 (NP_032904.1) (Met 1-Lys 491) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-491 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 0.5 nM as measured in 100μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7. 5.
• Molecular Mass: The secreted recombinant mouse Serpin F2 consists of 475 amino acids and has a calculated molecular mass of 53.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Serpinf2 serine (or cysteine) peptidase inhibitor, clade F, member 2 [ Mus musculus ]
• Official Symbol: Serpinf2
• Synonyms: SERPINF2; serine (or cysteine) peptidase inhibitor, clade F, member 2; alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-PI; alpha 2 antiplasmin; alpha-2-plasmin inhibitor; plasmin inhibitor alpha 2; pigment epithelium derived factor; serine (or cysteine) proteinase inhibitor, clade F, member 2; Pli; AI747498; Serpimf2
• Gene ID: 18816
• mRNA Refseq: NM_008878
• Protein Refseq: NP_032904