Recombinant Mouse Siglece protein, Fc-tagged
Cat.No. : | Siglece-057M |
Product Overview : | Recombinant Mouse Sialic Acid Binding Ig-like Lectin E is produced by our Mammalian expression system and the target gene encoding Gln20-Phe355 is expressed with a Fc tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Description : | Siglecs are sialic acid specific I-type lectins that are characterized by an extracellular domain (ECD) with an N-terminal Ig-like V-set domain followed by varying numbers of Ig-like C2-set domains. Mouse Siglec-E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 - 85 kDa member of the CD33-related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglec-9 is the most likely human ortholog of mouse Siglec-E. Siglec-E is expressed as a heavily N-glycosylated disulfide-linked homodimer and shows binding preference for disialic acids in the alpha 2-8 linkage. Siglec-E is up-regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglec-E signaling negatively regulates the LPS-induced production of TNF- alpha and IL-6 by macrophages. Its up-regulation in macrophages parallels the development of endotoxin tolerance. Siglec-E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL-12 p40 production. |
Source : | HEK293 |
Species : | Mouse |
Tag : | Fc |
Form : | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.5. |
Molecular Mass : | 85-95kDa, reducing conditions. |
Protein length : | Gln20-Phe355 |
Endotoxin : | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Purity : | Greater than 95% as determined by reducing SDS-PAGE. |
Storage : | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Reconstitution : | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Gene Name : | Siglece sialic acid binding Ig-like lectin E [ Mus musculus (house mouse) ] |
Official Symbol : | Siglece |
Synonyms : | SiglecE; Siglec-E; Sialic Acid Binding Ig-like Lectin E |
Gene ID : | 83382 |
mRNA Refseq : | NM_031181.2 |
Protein Refseq : | NP_112458.2 |
UniProt ID : | Q91Y57 |
Products Types
◆ Recombinant Protein | ||
Siglece-147M | Recombinant Mouse Siglece Protein, His (Fc)-Avi-tagged | +Inquiry |
Siglece-314M | Active Recombinant Mouse Siglece, Fc Chimera | +Inquiry |
SIGLECE-0696M | Recombinant Mouse SIGLECE protein, mFc-tagged | +Inquiry |
SIGLECE-15130M | Recombinant Mouse SIGLECE Protein | +Inquiry |
Siglece-6714M | Recombinant Mouse Siglece protein, His & S-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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