Active Recombinant Mouse Sirpa protein, His-tagged, Low Endotoxin

• Cat.No.: SIRPA-937M
• Product Overview: Recombinant Mouse Sirpa protein(BAA13520.1)(32-373 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 32-373 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse SIRP alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 44.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Sirpa signal-regulatory protein alpha [ Mus musculus ]
• Official Symbol: Sirpa
• Synonyms: SIRPA; signal-regulatory protein alpha; tyrosine-protein phosphatase non-receptor type substrate 1; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; SHP substrate 1; inhibitory receptor SHPS-1; signal-regulatory protein alpha-1; CD172 antigen-like family member A; brain immunological-like with tyrosine-based motifs; protein tyrosine phosphatase, non-receptor type substrate 1; brain Ig-like molecule with tyrosine-based activation motifs; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
• Gene ID: 19261
• mRNA Refseq: NM_001177646
• Protein Refseq: NP_001171117