"SIRPA" Related Products

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Recombinant Mouse SIRPA Protein, His-tagged

Cat.No. : SIRPA-937M
Product Overview : Recombinant extracellular domain of mouse SIRPA (BAA20376.1) (Met 1-Asn 373) was expressed, with a C-terminal polyhistidine tag.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Thr 32
Form : Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized mouse SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc. The EC50 of human CD47-Fc is 0.05-0.13 μg/ml.
Molecular Mass : The secreted recombinant mouse SIRPA comprises 353 amino acids and has a calculated molecular mass of 39.4 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA is approximately 50-70 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Sirpa signal-regulatory protein alpha [ Mus musculus ]
Official Symbol : SIRPA
Synonyms : SIRPA; signal-regulatory protein alpha; tyrosine-protein phosphatase non-receptor type substrate 1; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; SHP substrate 1; inhibitory receptor SHPS-1; signal-regulatory protein alpha-1; CD172 antigen-like family member
Gene ID : 19261
mRNA Refseq : NM_001177646
Protein Refseq : NP_001171117
MIM :
UniProt ID :
Pathway : Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem; Osteoclast differentiation, or
Function : SH3 domain binding; protein binding; protein phosphorylated amino acid binding;

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