Active Recombinant Mouse SIRPA protein, His-Avi-tagged, Biotinylated

• Cat.No.: SIRPA-0701M
• Product Overview: Biotinylated Recombinant Mouse SIRPA protein(Lys32-Asn373), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse SIRP alpha, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 41.5 kDa. The protein migrates as 55-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys32-Asn373
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sirpa signal-regulatory protein alpha [ Mus musculus ]
• Official Symbol: SIRPA
• Synonyms: SIRPA; signal-regulatory protein alpha; tyrosine-protein phosphatase non-receptor type substrate 1; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; SHP substrate 1; inhibitory receptor SHPS-1; signal-regulatory protein alpha-1; CD172 antigen-like family member A; brain immunological-like with tyrosine-based motifs; protein tyrosine phosphatase, non-receptor type substrate 1; brain Ig-like molecule with tyrosine-based activation motifs; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
• Gene ID: 19261
• mRNA Refseq: NM_001177646
• Protein Refseq: NP_001171117