Recombinant Mouse Smurf1 Protein, Myc/DDK-tagged

• Cat.No.: Smurf1-5984M
• Product Overview: Purified recombinant protein of mouse full-length SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
• Molecular Mass: 83.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smurf1 SMAD specific E3 ubiquitin protein ligase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Smurf1
• Synonyms: SMURF1; SMAD specific E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase SMURF1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; mKIAA1625; 4930431E10Rik
• Gene ID: 75788
• mRNA Refseq: NM_029438
• Protein Refseq: NP_083714
• UniProt ID: Q9CUN6