Recombinant Mouse Sparc protein, His-tagged

• Cat.No.: Sparc-7698M
• Product Overview: Recombinant Mouse Sparc aa. (Ala18~Ile302 (Accession # P07214)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Ala18~Ile302
• Form: Freeze-dried powder
• Molecular Mass: 33.8 kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in PBS, PH7.2-PH7.4.

Gene Information

• Gene Name: Sparc secreted acidic cysteine rich glycoprotein [ Mus musculus (house mouse) ]
• Official Symbol: Sparc
• Synonyms: ON; BM-40; basement-membrane protein 40; osteonectin; secreted protein acidic and rich in cysteine; SPARC
• Gene ID: 20692
• mRNA Refseq: NM_001290817.1
• Protein Refseq: NP_001277746.1
• UniProt ID: P07214