Recombinant Mouse Spop Protein, Myc/DDK-tagged

• Cat.No.: Spop-6105M
• Product Overview: Purified recombinant protein of mouse full-length speckle-type BTB/POZ protein (Spop), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. Involved in the regulation of bromodomain and extra-terminal motif (BET) proteins BRD2, BRD3, BRD4 stability.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 42.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Spop speckle-type BTB/POZ protein [ Mus musculus (house mouse) ]
• Official Symbol: Spop
• Synonyms: SPOP; speckle-type POZ protein; HIB homolog 1; PDX-1 C-terminal-interacting factor 1; TEF2; Pcif1; AI315626
• Gene ID: 20747
• mRNA Refseq: NM_025287
• Protein Refseq: NP_079563
• UniProt ID: Q6ZWS8