Recombinant Mouse SPP1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: SPP1-0598M
• Product Overview: Biotinylated Recombinant Mouse SPP1 protein(Leu17-Asn294), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 34.3 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu17-Asn294
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Spp1 secreted phosphoprotein 1 [ Mus musculus ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; osteopontin; minopontin; bone sialoprotein 1; osteopontin-like protein; 44 kDa bone phosphoprotein; early T-lymphocyte activation 1 protein; calcium oxalate crystal growth inhibitor protein; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
• Gene ID: 20750
• mRNA Refseq: NM_001204201
• Protein Refseq: NP_001191130