Active Recombinant Mouse Spp1 protein, His-tagged

• Cat.No.: SPP1-465M
• Product Overview: Recombinant Mouse Spp1 protein(Leu17-Asn294), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 17-294 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of B16 cells.When 5×10^4cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour.
• Molecular Mass: 31.59 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Spp1 secreted phosphoprotein 1 [ Mus musculus ]
• Official Symbol: Spp1
• Synonyms: SPP1; secreted phosphoprotein 1; osteopontin; minopontin; bone sialoprotein 1; osteopontin-like protein; 44 kDa bone phosphoprotein; early T-lymphocyte activation 1 protein; calcium oxalate crystal growth inhibitor protein; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
• Gene ID: 20750
• mRNA Refseq: NM_001204201
• Protein Refseq: NP_001191130