Recombinant Mouse Stx1a Protein, Myc/DDK-tagged

• Cat.No.: Stx1a-6205M
• Product Overview: Purified recombinant protein of mouse full-length syntaxin 1A (brain) (Stx1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Plays also an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 33.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Stx1a syntaxin 1A (brain) [ Mus musculus (house mouse) ]
• Official Symbol: Stx1a
• Synonyms: STX1A; syntaxin 1A (brain); syntaxin-1A; syntaxin 1 a; neuron-specific antigen HPC-1; HPC-1
• Gene ID: 20907
• mRNA Refseq: NM_016801
• Protein Refseq: NP_058081
• UniProt ID: O35526