Recombinant Mouse TGFB1 cell lysate

• Cat.No.: TGFB1-001MCL
• Product Overview: Mouse TGFβ1 / TGFB1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta 1; TGF-beta-1; regulatory protein; transforming growth factor-beta 1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• Gene ID: 21803
• mRNA Refseq: NM_011577
• Protein Refseq: NP_035707
• Pathway: Adipogenesis, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cell cycle signaling pathway, organism-specific biosystem
• Function: enzyme binding; eukaryotic cell surface binding; growth factor activity; protein N-terminus binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding

Reviews

11/16/2021

The impressive performance of the TGFB1 protein both in ELISA assays and protein electron microscopy structure analysis makes it a highly versatile and recommended choice for scientists across various disciplines.

03/01/2020

Its reliability and efficacy in generating high-quality data make it an indispensable tool in the quest for unraveling the mysteries of protein structure and function.

07/16/2018

The TGFB1 protein's excellent performance in ELISA ensures accurate and reproducible results, enhancing the reliability of experimental data.

Q&As

How is TGFB1 involved in bone remodeling?

TGFB1 stimulates the activity of cells involved in bone formation (osteoblasts) and plays a role in regulating bone resorption by influencing osteoclast function.

Are there any side effects associated with therapies targeting TGFB1?

Potential side effects of anti-TGFB1 therapies may include immune system suppression, gastrointestinal issues, and skin reactions.

Can TGFB1 be used as a biomarker for certain diseases?

TGFB1 levels have been studied as potential biomarkers for diseases like cancer and fibrosis, aiding in diagnosis and monitoring disease progression.

In what ways does TGFB1 influence wound healing?

TGFB1 plays a critical role in wound healing by stimulating the formation of new blood vessels, promoting the production of extracellular matrix, and aiding in the migration of cells to the wound site.

How does TGFB1 contribute to fibrosis?

TGFB1 can induce the production of excessive extracellular matrix proteins, leading to tissue scarring and fibrosis in organs such as the liver, lung, and kidneys.