Recombinant Mouse Trim21 Protein, His&Avi-tagged, Biotinylated

• Cat.No.: Trim21-7433MB
• Product Overview: Recombinant Mouse Trim21 Protein(NP_001076021.1) (Met1-Met462) was expressed in Insect Cells with a N-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: Avi&His
• Protein Length: 1-462 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20% Glycerol, 0.2 M Arg, 1 mM TCEP, pH 8.0.
• Bio-activity: 1.Loaded Biotinylated Recombinant Mouse TRIM21 Protein, His Tag on SA Biosensor, can bind Herceptin with an affinity constant of 4.58 nM as determined in BLI assay (Sartorius Octet RED384). 2.Loaded Biotinylated Recombinant Mouse TRIM21 Protein, His Tag on SA Biosensor, can bind Rituximab with an affinity constant of 8.76 nM as determined in BLI assay (Sartorius Octet RED384). 3.Loaded Biotinylated Recombinant Mouse TRIM21 Protein, His Tag on SA Biosensor, can bind OKT3 with an affinity constant of 17.6 nM as determined in BLI assay (Sartorius Octet RED384).
• Molecular Mass: The recombinant Mouse TRIM21 comprises 488 amino acids and has a predicted molecular mass of 56.65 kDa. It migrates as approximately 49.4 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 80 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Trim21 tripartite motif-containing 21 [ Mus musculus ]
• Official Symbol: Trim21
• Synonyms: TRIM21; tripartite motif-containing 21; E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; 52kD Ro/SSA autoantigen; Sjogren syndrome antigen A1; tripartite motif protein 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Ro52; Ssa1
• Gene ID: 20821
• mRNA Refseq: NM_001082552
• Protein Refseq: NP_001076021