Recombinant Mouse Tubb3 Protein, Myc/DDK-tagged

• Cat.No.: Tubb3-6728M
• Product Overview: Purified recombinant protein of mouse full-length tubulin, beta 3 class III (Tubb3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.
• Molecular Mass: 50.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tubb3 tubulin, beta 3 class III [ Mus musculus (house mouse) ]
• Official Symbol: Tubb3
• Synonyms: TUBB3; tubulin, beta 3 class III; tubulin beta-3 chain; betaIII-tubulin; M(beta)3; M(beta)6; 3200002H15Rik
• Gene ID: 22152
• mRNA Refseq: NM_023279
• Protein Refseq: NP_075768
• UniProt ID: Q9ERD7