Recombinant Mouse Txnrd1 Protein, Myc/DDK-tagged

• Cat.No.: Txnrd1-6754M
• Product Overview: Purified recombinant protein of mouse full-length thioredoxin reductase 1 (Txnrd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. [provided by RefSeq, May 2017]
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 55 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Txnrd1 thioredoxin reductase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Txnrd1
• Synonyms: TXNRD1; thioredoxin reductase 1; thioredoxin reductase 1, cytoplasmic; TR alpha; thioredoxin reductase TR1; TR; TR1; TrxR1
• Gene ID: 50493
• mRNA Refseq: NM_001042514
• Protein Refseq: NP_001035979
• UniProt ID: Q9JMH6