Recombinant Mouse Ube2a Protein, Myc/DDK-tagged

• Cat.No.: Ube2a-6779M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2A (Ube2a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 17.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2a ubiquitin-conjugating enzyme E2A [ Mus musculus (house mouse) ]
• Official Symbol: Ube2a
• Synonyms: UBE2A; ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae); ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; ubiquitin-conjugating enzyme HR6A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); HR6A; HHR6A; Mhr6a
• Gene ID: 22209
• mRNA Refseq: NM_019668
• Protein Refseq: NP_062642
• UniProt ID: Q9Z255