Recombinant Mouse Vtcn1 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Vtcn1-825MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant mature form of mouse B7H4 (Q7TSP5) (phe 29-Ser 256) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The secreted recombinant mouse B7H4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 469 amino acids and has a calculated molecular mass of 52.2 kDa. The apparent molecular mass of rmB7H4/Fc monomer is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: 469
• N-terminal Sequence Analysis: phe 29
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Vtcn1 V-set domain containing T cell activation inhibitor 1 [ Mus musculus ]
• Official Symbol: Vtcn1
• Synonyms: VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; t cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7x; B7h4; B7s1; BC032925; MGC41287
• Gene ID: 242122
• mRNA Refseq: NM_178594
• Protein Refseq: NP_848709