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Recombinant Mouse Vwf protein, His-tagged

Cat.No.: Vwf-1432M
Product Overview: Recombinant Mouse Vwf(Asp1498~Val1665) fused with His tag at N-terminal was expressed in E. coli.
Description: Vwf played an important role in many functions.
Source: E. coli
Species: Mouse
Tag : His
Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass: 20.4kDa
Protein length: Asp1498~Val1665
AA Sequence: DVV FVLEGSDEVG EANFNKSKEF VEEVIQRMDV SPDATRISVL QYSYTVTMEY AFNGAQSKEE VLRHVREIRY QGGNRTNTGQ ALQYLSEHSF SPSQGDRVEA PNLVYMVTGN PASDEIKRLP GDIQVVPIGV GPHANMQELE RISRPIAPIF IRDFETLPRE APDLV
Endotoxin: <1.0EU per 1µg (determined by the LAL method)
Purity: > 90%
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name: Vwf Von Willebrand factor homolog [ Mus musculus ]
Official Symbol: Vwf
Synonyms: VWF; Von Willebrand factor homolog; von Willebrand factor; VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik;
Gene ID: 22371
mRNA Refseq: NM_011708
Protein Refseq: NP_035838
UniProt ID: Q8CIZ8
Chromosome Location: 6 F3; 6 59.32 cM
Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Focal Adhesion, organism-specific biosystem;
Function: chaperone binding; collagen binding; glycoprotein binding; immunoglobulin binding; integrin binding; protease binding; protein N-terminus binding; protein binding; protein homodimerization activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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