Recombinant Mouse Wasl Protein, Myc/DDK-tagged

• Cat.No.: Wasl-6966M
• Product Overview: Purified recombinant protein of Mouse Wiskott-Aldrich syndrome-like (human) (Wasl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 54.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wasl WASP like actin nucleation promoting factor [ Mus musculus (house mouse) ]
• Official Symbol: Wasl
• Synonyms: WASL; Wiskott-Aldrich syndrome-like (human); neural Wiskott-Aldrich syndrome protein; N-WASP; 2900021I12Rik; 3110031I02Rik
• Gene ID: 73178
• mRNA Refseq: NM_028459
• Protein Refseq: NP_082735
• UniProt ID: Q91YD9