Recombinant Mouse Whamm Protein, Myc/DDK-tagged

• Cat.No.: Whamm-7000M
• Product Overview: Purified recombinant protein of Mouse WAS protein homolog associated with actin, golgi membranes and microtubules (Whamm), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Its activity in membrane tubulation requires F-actin and interaction with microtubules. Proposed to use coordinated actin-nucleating and microtubule-binding activities of distinct WHAMM molecules to drive membrane tubule elongation; when MT-bound can recruit and remodel membrane vesicles but is prevented to activate the Arp2/3 complex. Required for RhoD-dependent actin reorganization such as in cell adhesion and cell migration. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 89.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Whamm WAS protein homolog associated with actin, golgi membranes and microtubules [ Mus musculus (house mouse) ]
• Official Symbol: Whamm
• Synonyms: Whamm; WAS protein homolog associated with actin, golgi membranes and microtubules; Whdc1; BB081391; mKIAA1971; WASP homolog-associated protein with actin, membranes and microtubules; WAS protein homology region 2 domain containing 1; WAS protein homology region 2 domain-containing protein 1; WH2 domain-containing protein 1
• Gene ID: 434204
• mRNA Refseq: NM_001004185
• Protein Refseq: NP_001004185
• UniProt ID: Q571B6