Recombinant Mouse Wnk4 Protein, Myc/DDK-tagged

• Cat.No.: Wnk4-7004M
• Product Overview: Purified recombinant protein of Mouse WNK lysine deficient protein kinase 4 (Wnk4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 132.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wnk4 WNK lysine deficient protein kinase 4 [ Mus musculus (house mouse) ]
• Official Symbol: Wnk4
• Synonyms: WNK4; WNK lysine deficient protein kinase 4; serine/threonine-protein kinase WNK4; protein kinase with no lysine 4; protein kinase lysine-deficient 4; protein kinase, lysine deficient 4; Pha2b; Prkwnk4; 2010002J11Rik
• Gene ID: 69847
• mRNA Refseq: NM_175638
• Protein Refseq: NP_783569
• UniProt ID: Q80UE6