Recombinant Mouse YWHAE protein(Met1-Gln255), GST-tagged

• Cat.No.: YWHAE-888M
• Product Overview: Recombinant Mouse YWHAE (P62259) (Met 1-Gln 255) was expressed in E. coli, fused with the GST tag at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: N-GST
• Protein length: Met1-Gln255
• Form: Lyophilized from sterile 50mM Tris, 2mM GSH, 1mM DTT, pH 8.2. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ywhae tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide [ Mus musculus ]
• Official Symbol: YWHAE
• Synonyms: YWHAE; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; 14-3-3 protein epsilon; 14-3-3E; 14-3-3 epsilon; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; AU019196
• Gene ID: 22627
• mRNA Refseq: NM_009536
• Protein Refseq: NP_033562