"YWHAE" Related Products

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Recombinant Mouse YWHAE Protein, GST-tagged

Cat.No.: YWHAE-888M
Product Overview: Recombinant mouse YWHAE (P62259) (Met 1-Gln 255) was fused with the GST tag at the N-terminus.
Source: E. coli
Species: Mouse
Tag: GST
Predicted N Terminal: Met
Form: Lyophilized from sterile 50mM Tris, 2mM GSH, 1mM DTT, pH 8.21, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant mouse YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Ywhae tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide [ Mus musculus ]
Official Symbol: YWHAE
Synonyms: YWHAE; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; 14-3-3 protein epsilon; 14-3-3E; 14-3-3 epsilon; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; AU019196;
Gene ID: 22627
mRNA Refseq: NM_009536
Protein Refseq: NP_033562
MIM:
UniProt ID:
Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-spec
Function: enzyme binding; histone deacetylase binding; monooxygenase activity; phosphoprotein binding; phosphoserine binding; protein binding; protein complex binding; protein domain specific binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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