Recombinant Mouse Zap70 protein, His & T7-tagged

• Cat.No.: Zap70-1491M
• Product Overview: Recombinant Mouse Zap70 aa. (Ala270~Pro560 (Accession # P43404)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&T7
• Protein Length: Ala270~Pro560
• Description: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.0kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.1

Gene Information

• Gene Name: Zap70 zeta-chain (TCR) associated protein kinase [ Mus musculus (house mouse) ]
• Official Symbol: Zap70
• Synonyms: Zap70; zeta-chain (TCR) associated protein kinase; Srk; mur; mrtle; ZAP-70; tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; syk-related tyrosine kinase
• Gene ID: 22637
• mRNA Refseq: NM_001289612.1
• Protein Refseq: NP_001276541.1
• UniProt ID: P43404