Recombinant Murine Interleukin 12a, P35-P40

• Cat.No.: Il12a-211M
• Product Overview: Recombinant Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDA (p35) and 40 kDa (p40) subunits. It is expressed inCHO cells.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• Description: Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+ T cells and NK cells. Human IL-12 also promotes the development of proinflammatory/TH1-like CD4+ cells and cytotoxic CD8+T cells.
• Purity: Greater than 98% (determined by SDS-PAGE and HPLC analysis).
• Specific Activity: ≥ 1 x10⁷ IU/mg.
• Endotoxin Level: < 0.1 ng per µg of rMu IL-12 (1EU/µg).
• Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of INF-( production by murine splenocytes co-stimulated with IL-18 is ≤ 0.1 ng / ml, corresponding to a specific activity of ≥ 1 x10⁷ units/mg.
• Formulation: Lyophilized from a sterile-filtered protein solution in 0.5 x PBS (pH 7.2).
• Reconstitution: Please Note: Always centrifuge product before opening vial. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can than be diluted into other aqueous buffers and stored at -20°C for future use.
• Storage: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted murine IL-12 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus ]
• Synonyms: Il12a; interleukin 12a; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232; interleukin 12a p35 subunit; interleukin 12a
• Gene ID: 16159
• mRNA Refseq: NM_001159424
• Protein Refseq: NP_001152896
• UniProt ID: P43431
• Chromosome Location: 3 E1; 3 37.0 cM
• Pathway: Allograft rejection; Cytokine-cytokine receptor interaction; RIG-I-like receptor signaling pathway; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
• Function: cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding