Il12a-211M
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDA (p35) and 40 kDa (p40) subunits. It is expressed inCHO cells.
Description :
Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+ T cells and NK cells. Human IL-12 also promotes the development of proinflammatory/TH1-like CD4+ cells and cytotoxic CD8+T cells.
Source :
CHO cells.
Purity :
Greater than 98% (determined by SDS-PAGE and HPLC analysis).
Specific Activity :
≥ 1 x10⁷ IU/mg.
Endotoxin Level :
< 0.1 ng per µg of rMu IL-12 (1EU/µg).
Biological Activity :
The ED₅₀ as determined by the dose-dependent stimulation of INF-( production by murine splenocytes co-stimulated with IL-18 is ≤ 0.1 ng / ml, corresponding to a specific activity of ≥ 1 x10⁷ units/mg.
Formulation :
Lyophilized from a sterile-filtered protein solution in 0.5 x PBS (pH 7.2).
Reconstitution :
Please Note: Always centrifuge product before opening vial. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can than be diluted into other aqueous buffers and stored at -20°C for future use.
Storage :
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted murine IL-12 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.