Source : |
E. coli |
Species : |
M.viridifaciens |
Tag : |
His |
Predicted N Terminal : |
Met 1 |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is 10,000 pmoles/min/μg. as measured under the described conditions. |
Molecular Mass : |
The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted. |
Protein length : |
Pro42-Gly403 |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 97 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Official Symbol : |
NA |
Synonyms : |
neuraminidase;NA Protein |