| Source : |
Insect Cells |
| Tag : |
His |
| ProductOverview : |
Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques. |
| Description : |
ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. |
| AminoAcid Composition : |
ADPMTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFAHH HHHH |
| PhysicalAppearance : |
Liquid. |
| Purity : |
> 90% by SDS - PAGE. |
| Formulation : |
In 20mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol. |
| MolecularWeight : |
11 kDa (104aa) |
| Concentration : |
0.5 mg/ml (determined by Bradford assay) |
| Application : |
SDS-PAGE |
| Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References : |
Pathak SK., et al. (2007) Natlmmunol. 8(6):610-8.Matveeva NA., et al. (2011) Tsitol Genet. 45(1):11-7. |
