| Species : |
Oryza sativa |
| Source : |
Pichia pastoris |
| Tag : |
Non |
| Description : |
PNGase A cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without α(1,3)-linked core fucose residues.
Cleaves N-glycans from plant and insect-derived glycoproteins and glycopeptides
Activity is not inhibited by an α1-3 Fucose residue on the chitobiose core
Recombinant enzyme with no detectable exoglycosidase or other endoglycosidase contaminating activities
Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
≥95% purity, as determined by SDS-PAGE and intact ESI-MS
Optimal activity and stability for up to 24 months |
| Molecular Mass : |
63.8 kDa |
| Unit Definition : |
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 1 μg of denatured recombinant Avidin produced in Maize in 1 hour at 37 centigrade in a total reaction volume of 10 μL. |
| Unit Assay Conditions : |
1 μg of recombinant Avidin is denatured with 1X Glycoprotein Denaturing Buffer at 100 centigrade for 10 minutes. After the addition of NP-40 and GlycoBuffer 3, two-fold dilutions of PNGase A are added and the reaction mix is incubated for 1 hour at 37 centigrade. Separation of reaction products are visualized by SDS-PAGE. |
| Heat Inactivation : |
65 centigrade for 10 minutes |
| Storage Buffer : |
20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5 at 25 centigrade |
