Recombinant Pig BDNF protein, His-tagged BDNF-551P

Recombinant Pig BDNF protein, His-tagged

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Recombinant Pig BDNF protein, His-tagged

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Cat.No. : BDNF-551P
Product Overview : Recombinant Pig BDNF (P14082) aa. (Pro20~Arg252) fused with N-terminal His tag was produced in E. coli cells.
Source : E. coli
Species : Pig
Tag : His
Form : Freeze-dried powder
Molecular Mass : 32kDa as determined by SDS-PAGE reducing conditions.
Protein length : Pro20~Arg252
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Characteristic : The isoelectric point is 8.8.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200µg/mL
Storage buffer : PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Reconstitution : Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : BDNF brain derived neurotrophic factor [ Sus scrofa (pig) ]
Official Symbol : BDNF
Synonyms : BDNF; brain derived neurotrophic factor; brain-derived neurotrophic factor
Gene ID : 397495
mRNA Refseq : NM_214259.2
Protein Refseq : NP_999424.1
UniProt ID : P14082

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