Recombinant Pig CYP2C49 Protein

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Recombinant Pig CYP2C49 Protein

CYP2C49 Related Products

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Cat.No. : CYP2C49-131P
Product Overview : Recombinant pig CYP2C49 protein and pig CYP-reductase without tag were coexpressed in E. coli.
Description : Biased expression in liver (RPKM 449.8), mixtures (RPKM 159.5) and 1 other tissu
Source : E. coli
Species : Pig
Bio-activity : Cytochrome c Reductase Activity: 303 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Tolbutamide 4-hydroxylase
Linear with time up to: 60 min(Determined at 350 µM substrate, using a P450 concentration of 100 pmol/ml)
Linear with P450 up to: 120 pmol/ml(Determined at 350 µM substrate, using an incubation time of 20 min)
Vmax: 0.41 pmol/min/pmol CYP
Km: 586 µM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 10.0 nmol/ml
Protein Concentration: 15.5 mg/ml
Specific P450 Content: 646 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP2C49 cytochrome P450 2C49 [ Sus scrofa (pig) ]
Official Symbol : CYP2C49
Synonyms : CYP2C49; cytochrome P450 2C49; cytochrome P450 2C49
Gene ID : 403215
mRNA Refseq : NM_214420
Protein Refseq : NP_999585
UniProt ID : Q8SQ65

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