Recombinant Pig CYP2C49 Protein CYP2C49-131P

Specification Gene Information

Cat.No. :	CYP2C49-131P
Product Overview :	Recombinant pig CYP2C49 protein and pig CYP-reductase without tag were coexpressed in E. coli.
Description :	Biased expression in liver (RPKM 449.8), mixtures (RPKM 159.5) and 1 other tissu
Source :	E. coli
Species :	Pig
Bio-activity :	Cytochrome c Reductase Activity: 303 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: Tolbutamide 4-hydroxylase Linear with time up to: 60 min(Determined at 350 µM substrate, using a P450 concentration of 100 pmol/ml) Linear with P450 up to: 120 pmol/ml(Determined at 350 µM substrate, using an incubation time of 20 min) Vmax: 0.41 pmol/min/pmol CYP Km: 586 µM
Usage :	For laboratory (research) purposes only
Storage :	Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration :	P450 Concentration: 10.0 nmol/ml Protein Concentration: 15.5 mg/ml Specific P450 Content: 646 pmol/mg protein
Storage Buffer :	50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Name :	CYP2C49 cytochrome P450 2C49 [ Sus scrofa (pig) ]
Official Symbol :	CYP2C49
Synonyms :	CYP2C49; cytochrome P450 2C49; cytochrome P450 2C49
Gene ID :	403215
mRNA Refseq :	NM_214420
Protein Refseq :	NP_999585
UniProt ID :	Q8SQ65

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