Active Recombinant Pig CYP2C49 Protein

• Cat.No.: CYP2C49-131P
• Product Overview: Recombinant pig CYP2C49 protein and pig CYP-reductase without tag were coexpressed in E. coli.

Specification

• Species: Pig
• Source: E.coli
• Description: Biased expression in liver (RPKM 449.8), mixtures (RPKM 159.5) and 1 other tissu
• Bio-activity: Cytochrome c Reductase Activity: 303 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Tolbutamide 4-hydroxylase
  Linear with time up to: 60 min(Determined at 350 µM substrate, using a P450 concentration of 100 pmol/ml)
  Linear with P450 up to: 120 pmol/ml(Determined at 350 µM substrate, using an incubation time of 20 min)
  Vmax: 0.41 pmol/min/pmol CYP
  Km: 586 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 10.0 nmol/ml
  Protein Concentration: 15.5 mg/ml
  Specific P450 Content: 646 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C49 cytochrome P450 2C49 [ Sus scrofa (pig) ]
• Official Symbol: CYP2C49
• Synonyms: CYP2C49; cytochrome P450 2C49; cytochrome P450 2C49
• Gene ID: 403215
• mRNA Refseq: NM_214420
• Protein Refseq: NP_999585
• UniProt ID: Q8SQ65