Recombinant Pig CYP3A39 Protein CYP3A39-133P

Specification Gene Information

Cat.No. :	CYP3A39-133P
Product Overview :	Recombinant pig CYP3A39 protein and CYP-reductase without tag were coexpressed in E. coli.
Description :	Cytochromes P450 are a group of heme-thiolate monooxygenases.
Source :	E. coli
Species :	Pig
Bio-activity :	Cytochrome c Reductase Activity: 200 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: Testosterone 6β-hydroxylase Linear with time up to: 30 min(Determined at 32 µM substrate, using a P450 concentration of 175 pmol/ml) Linear with P450 up to: 250 pmol/ml(Determined at 32 µM substrate, using an incubation time of 20 min) Vmax: 0.41 pmol/min/pmol CYP Km: 58 µM
Usage :	For laboratory (research) purposes only
Storage :	Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration :	P450 Concentration: 9.9 nmol/ml Protein Concentration: 19.5 mg/ml Specific P450 Content: 508 pmol/mg protein
Storage Buffer :	50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Name :	CYP3A39 cytochrome P450 3A39 [ Sus scrofa (pig) ]
Official Symbol :	CYP3A39
Synonyms :	CYP3A39; cytochrome P450 3A39; CYP3A22; cytochrome P450 3A39; cytochrome P450 3A22
Gene ID :	403322
mRNA Refseq :	NM_214422
Protein Refseq :	NP_999587
UniProt ID :	O97689

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