Active Recombinant Pig CYP3A39 Protein

• Cat.No.: CYP3A39-133P
• Product Overview: Recombinant pig CYP3A39 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Species: Pig
• Source: E.coli
• Description: Cytochromes P450 are a group of heme-thiolate monooxygenases.
• Bio-activity: Cytochrome c Reductase Activity: 200 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 6β-hydroxylase
  Linear with time up to: 30 min(Determined at 32 µM substrate, using a P450 concentration of 175 pmol/ml)
  Linear with P450 up to: 250 pmol/ml(Determined at 32 µM substrate, using an incubation time of 20 min)
  Vmax: 0.41 pmol/min/pmol CYP
  Km: 58 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 9.9 nmol/ml
  Protein Concentration: 19.5 mg/ml
  Specific P450 Content: 508 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP3A39 cytochrome P450 3A39 [ Sus scrofa (pig) ]
• Official Symbol: CYP3A39
• Synonyms: CYP3A39; cytochrome P450 3A39; CYP3A22; cytochrome P450 3A39; cytochrome P450 3A22
• Gene ID: 403322
• mRNA Refseq: NM_214422
• Protein Refseq: NP_999587
• UniProt ID: O97689