Active Recombinant Rabbit IL4 protein, His-tagged

Name: Active Recombinant Rabbit IL4 protein, His-tagged
SKU: IL4-1040R

Species :	Rabbit
Source :	HEK293
Tag :	His
Protein Length :	26-147 aa
Form :	Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity :	The interleukin 4 (IL4,) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. The activity of IL4 is usually measured by a cell proliferation assay using TF-1 cells. TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20000 cells/well with 10% serum standard 1640 which contains various concentrations of recombinant rabbit IL4. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37°C. Proliferation of TF-1 cells after incubation with IL4 for 3 days observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with IL4 for 3 days. It was obvious that recombinant rabbit IL4 significantly increased cell viability of TF-1 cells. The ED50 is 0.22 ug/ml.
Molecular Mass :	15.6kDa
Endotoxin :	<1.0EU per 1μg (determined by the LAL method).
Purity :	> 90% as determined by SDS-PAGE.
Storage :	Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution :	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Name	IL4 interleukin 4 [ Oryctolagus cuniculus ]
Official Symbol	IL4
Synonyms	IL4; interleukin 4; interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; IL2; BSF-1;
Gene ID	100302454
mRNA Refseq	NM_001163177
Protein Refseq	NP_001156649