Recombinant Rat Adamts5 protein, His & T7-tagged

• Cat.No.: Adamts5-1756R
• Product Overview: Recombinant Rat Adamts5 aa. (Arg267~Asn622 (Accession # Q6TY19)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Human homolog catalyzes the degradation of aggrecan, a component of cartilage extracellular matrix.
• Source: E. coli
• Species: Rat
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.9kDa
• Protein length: Arg267~Asn622 (Accession # Q6TY19)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 7.3
• Publication:
  Loss of TIMP3 expression induces inflammation, matrix degradation, and vascular ingrowth in nucleus pulposus: A new mechanism of intervertebral disc degeneration (2020)

Gene Information

• Gene Name: Adamts5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Adamts5
• Synonyms: Adamts5; ADAM metallopeptidase with thrombospondin type 1 motif, 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
• Gene ID: 304135
• mRNA Refseq: NM_198761.1
• Protein Refseq: NP_942056.1
• UniProt ID: Q6TY19